Journal: Frontiers in Immunology

Article Title: Allergic T H 2 Response Governed by B-Cell Lymphoma 6 Function in Naturally Occurring Memory Phenotype CD4 + T Cells

doi: 10.3389/fimmu.2018.00750

Figure Lengend Snippet: Role of Bcl6-mediated MPT cell functions in NAT H 2 differentiation in an allergic murine model. [ (A) top] Mixture of purified KJ1-26 − MPT cells ( Bcl6 -WT or Bcl6 -KO) and KJ1-26 + WT naïve CD4 + T cells were transferred into BALB/c nu/nu mice intravenously (day 0). These mice were immunized with alum-conjugated OVA and then intratracheally challenged with OVA. [ (A) bottom] Absolute cell numbers of Neu, Eos, AM, and Lym in BALF, (B) hematoxylin and eosin-stained, formalin-fixed lung sections (magnification: 200×), and (C) T H 2 cytokine levels in the BALF of recipient mice 48 h after the last OVA challenge. (D) Relative Il4, Il5 , and Il13 expression mRNA in splenic KJ1-26 + T cells restimulated with anti-CD3 monoclonal antibodies 5 days after the last challenge. (E) OVA-specific IgE antibody titers in sera from each recipient of Bcl6 -WT NAT H 2 cells, plus MPT H 2 cells transferred from Bcl6 -TG, Bcl6 -WT, or Bcl6 -KO mice 2 days after the last challenge. All results are representative of four independent experiments with similar outcomes. Data are presented as the mean ± SEM ( n = 5–7). * P < 0.05, ** P < 0.01, comparison between two groups is indicated. AM, alveolar macrophages; BALF, bronchoalveolar lavage fluid; Bcl6, B-cell lymphoma 6; Eos, eosinophils; KJ + , KJ1-26-positive; KO, knockout; Lym, lymphocytes; MPT cell, memory phenotype CD4 + T cell; MPT H 2 cell, MPT cell-derived T H 2 cell; NAT H 2 cell; naïve CD4 + T cell-derived T H 2 cell; Neu, neutrophils; NS, not significant; OVA, ovalbumin; TG, transgenic; WT, wild-type.

Article Snippet: IgE anti-OVA Abs were detected using a mouse anti-OVA IgE Antibody Assay Kit (Chondrex, Redmond, WA, USA).

Techniques: Purification, Staining, Expressing, Knock-Out, Derivative Assay, Transgenic Assay

Journal: The World Allergy Organization Journal

Article Title: High protein diet increases the risk of allergic sensitization but not asthma in mice through modulation of the cytokine milieu toward Th2 bias ☆

doi: 10.1016/j.waojou.2025.101031

Figure Lengend Snippet: Effect of feeding mice for 7 weeks either normal diet or high protein diet on serum levels of (A) OVA-specific IgE (B) OVA-specific IgG1 and (C) OVA-specific IgG2a from OVA-sensitized mice (n = 11 in each group) after OVA intranasal challenge. The high protein diet group showed higher levels of both OVA-specific IgE and IgG1compared to the normal diet group. ns: not significant, CI: confidence interval. IQR: interquartile range.

Article Snippet: After washing the wells, blocking buffer (0.5% gelatin in wash buffer or 3% Rat serum in wash buffer) was added and the plates incubated for 2 h. Appropriate reference standard OVA-IgE (Chondrex #3006) or OVA-IgG1 (Chondrex # 7093) or OVA-IgG2a (Chondrex # 7095) and diluted serum samples were then added to the wells and incubated for 3 h. This was followed by incubation with OVA-Biotin for 1 h and with streptavidin-HRP for 30 min. Tetramethylbenzidine substrate was added and the enzyme-substrate reaction stopped after 15 min, and the optical density measured at 450 nm.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Effects of Oral Exposure to Low-Dose Bisphenol S on Allergic Asthma in Mice

doi: 10.3390/ijms231810790

Figure Lengend Snippet: Serum levels of OVA-specific immunoglobulin (Ig) antibodies. OVA-specific IgE and IgG 1 were measured 48 h after the last intratracheal administration by ELISA. ( a , b ) OVA-IgE. ( c , d ) OVA-IgG 1 . Data are expressed as mean ± SE for 5–6 animals per group. # p < 0.05 vs. OVA group. ELISA, enzyme-linked immunosorbent assay; OVA, ovalbumin; SE, standard error.

Article Snippet: Furthermore, OVA-specific IgE and IgG 1 were measured using mouse anti-OVA-IgE enzyme-linked immunosorbent assay (ELISA) Kit (Shibayagi Co., Gunma, Japan) and anti-OVA-IgG 1 ELISA Kit (Shibayagi Co.) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay